Department of Defense installations: Avon Park Air Force Range, Cape Canaveral Air Station, Eglin Air Force Base, Eglin Air Force Base Test Site, and (part of) Tyndall Air Force Base

National Estuarine Research Reserve: Rookery Bay

National Forests: Apalachicola, Ocala, and Osceola

National Monument: Fort Matanzas

National Seashores: Canaveral, and Gulf Islands

National Parks: Biscayne, Dry Tortugas, and Everglades

National Preserve: Big Cypress

National Wildlife Refuges: Arthur R. Marshall Loxahatchee, Cedar Keys, Chassahowitzka, Crocodile Lake, Florida Panther, Great White Heron, J.N. "Ding" Darling, Key West, Lake Wales Ridge, Lake Woodruff, Lower Suwannee, Merritt Island, National Key Deer, Okefenokee, Passage Key, Pelican Island, Pine Island, Pinellas, St. Johns, St. Marks, St. Vincent, and Ten Thousand Islands

Other: Kingsley Plantation, and Timucuan Ecological and Historic Preserve

Fish Management Area: Tenoroc

Military Reservation: Cape Blanding

Northwest Florida Water Management District properties: [none yet]

St. Johns Water Management District Conservation Areas: Blue Cypress, Buck Lake, Canaveral Marshes, Emeralda Marsh, Fort Drum Marsh, Lake George, Lochloosa Wildlife, Moses Creek, Seminole Ranch, and Three Forks Marsh

St. Johns Water Management District other properties: Brevard Coastal Scrub Ecosystem, Gum Root Swamp, Lake Apopka Restoration Area, and Ranch Reserve

South Florida Water Management District properties: Corkscrew Regional Ecosystem Watershed, Corkscrew Regional Mitigation Bank, Dupuis Reserve, East Coast Buffer, Frog Pond/L-31 N Transitional Lands, Kissimmee Chain of Lakes, Kissimmee River, Loxahatchee Slough, Model Lands Basin, Pal–Mar, Southern Glades, Stormwater Treatment Areas, Strazzulla Tract, and Upper Lakes Basin Watershed

Southwest Florida Water Management District properties: Annutteliga Hammock, Bright Hour Watershed, Chassahowitzka River and Coastal Swamps, Cypress Creek Flood Detention Area, Flying Eagle Ranch, Green Swamp Wilderness, Gum Slough, Halpata Tastanaki Preserve, Jack Creek, Lake Panasoffkee, Myakka River, Panasoffkee/Outlet Tract, Potts Preserve, Starkey Wilderness Park, and Weekiwachee Preserve

State Forests: Blackwater River, Goethe, Jennings, Lake George, Lake Wales Ridge, Myakka, Picayune Strand, Ross Prairie, Seminole, Tate’s Hell, and Withlacoochee

State Parks: Allen David Broussard Catfish Creek Preserve, Anastasia, Anclote Key Preserve, Bahia Honda, Big Talbot Island, Bill Baggs Cape Florida, Caladesi Island, Collier–Seminole, Curry Hammock, Dr. Julian G. Bruce St. George Island, Edward Ball Wakulla Springs, Fakahatchee Strand Preserve, Faver–Dykes, Fort Clinch, Fort George Island Cultural, Guana River, Highlands Hammock, Honeymoon Island, Hugh Taylor Birch, Ichetucknee Springs, John Pennekamp Coral Reef, John U. Lloyd Beach, Jonathan Dickinson, Key Largo Hammock Botanical, Kissimmee Prairie Preserve, Lake June-In-Winter Scrub, Lake Kissimmee, Lake Louisa, Lignumvitae Key Botanical, Little Talbot Island, Long Key, Lower Wekiva River Preserve, Myakka River, Oscar Scherer, Paynes Prairie Preserve, San Felasco Hammock Preserve, (part of) St. Andrews, T.H. Stone Memorial St. Joseph Peninsula, Tomoka, Topsail Hill Preserve, Waccasassa Bay Preserve, Wekiwa Springs, and Werner–Boyce Salt Springs

State Recreation and Conservation Area: (parts of) Cross Florida Greenway

State Reserves: Cape St. George, Cedar Key Scrub, Rock Springs Run, and William Beardall Tosohatchee

Suwannee Water Management District properties: [none yet]

Wildlife and Environmental Areas: Florida Keys, Lake Placid, Little Gator Creek, Platt Branch Mitigation Park, and Split Oak Mitigation Park

Wildlife Management Areas: Big Bend, Bull Creek, Chassahowitzka, Everglades and Francis S. Taylor, Fisheating Creek, Fred C. Babcock–Cecil M. Webb, Guana River, Half Moon, Hilochee, Holey Land, J.W. Corbett, Rotenberger, Three Lakes, and Triple N Ranch

Alachua County: Gum Root Park/Gum Root Conservation Area

Brevard County: Batchelor, Dicerandra Scrub Sanctuary, Enchanted Forest Sanctuary, Jordan Boulevard, Malabar Scrub Sanctuary, Micco Scrub Sanctuary, North Rockledge Sanctuary, South Babcock/Ten Mile Ridge, Tico Scrub Sanctuary, Turkey Creek Sanctuary, and Valkaria Scrub Sanctuary

Duval County: Huguenot Memorial Park

Hillsborough County: E.G. Simmons Park, The Kitchen, and Wolf Branch

Lee County: Stairstep Mitigation Bank

Miami-Dade County: Charles Deering Estate, and Matheson Hammock Park

Orange County: Moss Park

Osceola County: Lake Lizzie Nature Preserve

Palm Beach County: City of West Palm Beach Water Catchment Area, Loxahatchee River Natural Area, Loxahatchee Slough Natural Area, and Pal–Mar Natural Area

Pasco County: Withlacoochee River Park

Pinellas County: Al-Bar Ranch, Cross Bar Ranch Wellfield, and Fort De Soto County Park

Polk County: Babson/Hesperides Tract, Saddle Creek County Park, and Sumica/Lake Walk-in-the-Water Tract

Sarasota County: North Lido Public Beach, Palmer Point County Park, Pinelands Reserve, and T. Mabry Carleton, Jr. Memorial Reserve

Volusia County: Smyrna Dunes Park

Archbold Expeditions, Inc.: Archbold Biological Station

Audubon of Florida: Corkscrew Swamp Sanctuary, Florida Coastal Islands Sanctuaries, Lake Okeechobee Sanctuaries, Sabal Point Sanctuary, and Saddle Creek Sanctuary

Tall Timbers Research Station: Tall Timbers Research Station, and the following Conservation Easements: Allee Property, Chemonie Plantation, Conlin Island, Foshalee Plantation, Horseshoe Plantation, Pickney Hill Plantation, Straw Pond, Sunny Hill Plantation, Swamp Creek Preserve, and Woodfield Springs Plantation

The Nature Conservancy: Avalon Plantation Conservation Easement, Carter Creek, Catfish Creek, Disney Wilderness Preserve, Fillman Bayou Preserve, Holmes Avenue, Jeff Lewis Wilderness Preserve, Mays Pond Plantation Conservation Easement, Saddle Blanket Lakes Preserve, Saddle Bunch Keys, Sun Ray Scrub, Tiger Creek Preserve, Torchwood Hammock Preserve, and Withlacoochee Swamp Conservation Easement